Journal: PLoS ONE

Article Title: Loss of Grainy Head-Like 1 Is Associated with Disruption of the Epidermal Barrier and Squamous Cell Carcinoma of the Skin

doi: 10.1371/journal.pone.0089247

Figure Lengend Snippet: (A) Blood levels of TSLP in Grhl1 +/+ (gray bar) and Grhl1 −/− mice (blue bar), measured with ELISA kit. (B) Levels of expression of antimicrobial peptides S100A8 and S100A9 in the epidermis of Grhl1 +/+ (gray bar) and Grhl1 −/− mice (blue bar), measured with Q-RT-PCR. (C) Representative skin sections of Grhl1 +/+ (top panel) and Grhl1 −/− mice (bottom panel) stained with toluidine blue to visualize dermal mast cells (purple cells). Scale bars represent 200 µm. (D) Quantification of skin infiltration with mast cells for Grhl1 +/+ (gray bar) and Grhl1 −/− mice (blue bar), estimated as numbers of stained cells per 1 mm 2 area of 10 µm thick skin section (using ImageJ software). (A, B, D) Significance (Student’s t-test, p-value) is shown above bars.

Article Snippet: The blood level of TSLP was measured using enzyme-linked immunosorbent assay (ELISA) kit: Quantikine Mouse TSLP (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions, on groups of seven Grhl1 −/− and five Grhl1 +/+ animals, at the age of about 4 months.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Staining, Software

Journal: Cells

Article Title: Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients

doi: 10.3390/cells14090654

Figure Lengend Snippet: The concentration of IL-6, TSLP, and TGF- β in plasma. Data presented as the mean ± SD. CRSsNP—chronic rhinosinusitis without nasal polyposis; CRSwNP—chronic rhinosinusitis with nasal polyposis; IL-6—interleukin 6; TGF- β —transforming growth factor β ; TSLP—thymic stromal lymphopoietin. CRSsNP n = 15; CRSwNP n = 15; control group n = 10. Lines connect comparison groups with a p -value denoting the significant difference and pair-wise comparisons. Statistical analysis between investigated groups—two-sided Mann–Whitney U test (independent data).

Article Snippet: For ELISA following reagents were used: Human IL-6 ELISA kit (Catalog No: BSKH1007 Bioss Inc., Woburn, MA, USA); Human TSLP ELISA kit (Catalog No: BSKH1031 Bioss Inc., Woburn, MA, USA); Human TGF- β 1 ELISA kit (Catalog No: BSKH60051 Bioss Inc., Woburn, MA, USA).

Techniques: Concentration Assay, Clinical Proteomics, Control, Comparison, MANN-WHITNEY

Journal: Cells

Article Title: Gene Expression of Extracellular Matrix Proteins, MMPs, and TIMPs in Post-Operative Tissues of Chronic Rhinosinusitis Patients

doi: 10.3390/cells14090654

Figure Lengend Snippet: ( A ) Correlation between eosinophil count and SNOT-22 score in CRSwNP group; ( B ) correlation between fibronectin gene expression and WBC count in CRSwNP group; ( C ) correlation between periostin gene expression and TSLP level in CRSwNP group; ( D ) correlation between collagen I gene expression and TSLP level in CRSwNP group; ( E ) correlation between collagen III gene expression and TSLP level in CRSwNP group; ( F ) correlation between TIMP-2 gene expression and SNOT-22 score in CRSwNP group; ( G ) correlation between fibronectin gene expression and SNOT-22 score in CRSwNP group. SNOT-22—sinonasal outcome test; TIMP-2—tissue inhibitor of metalloproteinases 2; TSLP thymic stromal lymphopoietin; WBC—white blood cells. Gene expression data are presented as ΔΔCt, with lower ΔΔCt values indicating higher gene expression. CRSwNP n = 15. Statistical analysis—Spearman r test and nonlinear regression.

Article Snippet: For ELISA following reagents were used: Human IL-6 ELISA kit (Catalog No: BSKH1007 Bioss Inc., Woburn, MA, USA); Human TSLP ELISA kit (Catalog No: BSKH1031 Bioss Inc., Woburn, MA, USA); Human TGF- β 1 ELISA kit (Catalog No: BSKH60051 Bioss Inc., Woburn, MA, USA).

Techniques: Gene Expression

Journal: Scientific Reports

Article Title: Epithelial-interleukin-1 inhibits collagen formation by airway fibroblasts: Implications for asthma

doi: 10.1038/s41598-020-65567-z

Figure Lengend Snippet: Production of IL-1 & IL-33 in differentiated air-liquid interface (ALI) cultures of primary airway epithelial cells. Primary airway epithelial cells (PAECs) from non-asthmatics (n = 5) and asthmatics (n = 10) were cultured at an air-liquid interface, RNA and supernatants were collected at Days (D) 1, 5, 11 and 20. ( a ) IL-1α ( b ) IL-1β and ( c ) IL-33 expression from PAECs are expressed as normalized to base pair reads. The concentration of ( d ) IL-1α & ( e ) IL-1β released from PAECs was measured by ELISA. Means ± SEM are shown. Exact P values indicated.

Article Snippet: Cell-culture supernatants were also harvested on the same days and samples were assessed in duplicate via the ELISA kits for IL-1α, IL-1β, IL-33, IL-6, IL-8, GM-CSF and TSLP (R&D Systems, Minneapolis) according to manufacturer instructions and the concentration (pg/ml) normalized to total protein content (pg/mg of total protein) assessed using the Pierce Rapid Gold BCA Protein Assay Kit (Thermofisher).

Techniques: Cell Culture, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Epithelial-interleukin-1 inhibits collagen formation by airway fibroblasts: Implications for asthma

doi: 10.1038/s41598-020-65567-z

Figure Lengend Snippet: IL-1 but not IL-33 stimulates the release of inflammatory mediators from primary airway fibroblasts (PAFs). Primary airway fibroblasts from non-asthmatics (n = 5–9) and asthmatics (n = 5–9) were grown to confluence on collagen I coated plates and stimulated with or without 1 ng/ml recombinant human IL-1α, IL-1β or IL-33 for 24 hours. Concentration of ( a ) IL-6, ( b ) CXCL8/IL-8, ( c ) granulocyte-monocyte colony stimulating factor (GM-CSF) & ( d ) thymic stromal lymphopoietin (TSLP) released from primary airway fibroblasts after 24 hours. Exact P values indicated.

Article Snippet: Cell-culture supernatants were also harvested on the same days and samples were assessed in duplicate via the ELISA kits for IL-1α, IL-1β, IL-33, IL-6, IL-8, GM-CSF and TSLP (R&D Systems, Minneapolis) according to manufacturer instructions and the concentration (pg/ml) normalized to total protein content (pg/mg of total protein) assessed using the Pierce Rapid Gold BCA Protein Assay Kit (Thermofisher).

Techniques: Recombinant, Concentration Assay